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cd80- pecy5  (Thermo Fisher)


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    Thermo Fisher cd80- pecy5
    A) The percentage of CD11c+/Siglec F−/MHC IIhi total lung DCs and B) the percentage of lung DC monocyte-derived dendritic cells (moDCs), conventional myeloid dendritic cells (cDCs), CD103+ DCs, and plasmacytoid dendritic cells (pDCs) subsets as quantified by flow cytometry (P = NS, HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice, n= 9 mice per group, unpaired t test). C) Cell surface expression of <t>CD80</t> and CD86 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 8 – 9 mice per group, * P< 0.01, Mann-Whitney test). D) Cell surface expression of Dectin1, Dectin 2, CD205, CD206, and DC-SIGN by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 4 – 9 mice per group, * P = 0.029, Mann-Whitney test). E) Cell surface expression of TLR4 and TLR5 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n= 4 – 9 mice per group, *P < 0.05, unpaired t test).
    Cd80 Pecy5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd80-+pecy5/pmc06013321-175-37-44
    Average 90 stars, based on 1 article reviews
    cd80- pecy5 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "LRP-1 Attenuates House Dust Mite-induced Eosinophilic Airway Inflammation by Suppressing Dendritic Cell-mediated Adaptive Immune Responses"

    Article Title: LRP-1 Attenuates House Dust Mite-induced Eosinophilic Airway Inflammation by Suppressing Dendritic Cell-mediated Adaptive Immune Responses

    Journal: The Journal of allergy and clinical immunology

    doi: 10.1016/j.jaci.2017.10.044

    A) The percentage of CD11c+/Siglec F−/MHC IIhi total lung DCs and B) the percentage of lung DC monocyte-derived dendritic cells (moDCs), conventional myeloid dendritic cells (cDCs), CD103+ DCs, and plasmacytoid dendritic cells (pDCs) subsets as quantified by flow cytometry (P = NS, HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice, n= 9 mice per group, unpaired t test). C) Cell surface expression of CD80 and CD86 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 8 – 9 mice per group, * P< 0.01, Mann-Whitney test). D) Cell surface expression of Dectin1, Dectin 2, CD205, CD206, and DC-SIGN by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 4 – 9 mice per group, * P = 0.029, Mann-Whitney test). E) Cell surface expression of TLR4 and TLR5 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n= 4 – 9 mice per group, *P < 0.05, unpaired t test).
    Figure Legend Snippet: A) The percentage of CD11c+/Siglec F−/MHC IIhi total lung DCs and B) the percentage of lung DC monocyte-derived dendritic cells (moDCs), conventional myeloid dendritic cells (cDCs), CD103+ DCs, and plasmacytoid dendritic cells (pDCs) subsets as quantified by flow cytometry (P = NS, HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice, n= 9 mice per group, unpaired t test). C) Cell surface expression of CD80 and CD86 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 8 – 9 mice per group, * P< 0.01, Mann-Whitney test). D) Cell surface expression of Dectin1, Dectin 2, CD205, CD206, and DC-SIGN by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 4 – 9 mice per group, * P = 0.029, Mann-Whitney test). E) Cell surface expression of TLR4 and TLR5 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n= 4 – 9 mice per group, *P < 0.05, unpaired t test).

    Techniques Used: Derivative Assay, Flow Cytometry, Expressing, MANN-WHITNEY

    Related Articles

    Derivative Assay:

    Article Title: LRP-1 Attenuates House Dust Mite-induced Eosinophilic Airway Inflammation by Suppressing Dendritic Cell-mediated Adaptive Immune Responses
    Article Snippet: Mouse DC subsets were identified and characterized by using anti- mouse Siglec F-PE-CF594, CD11c-APC Cy7, MHCII-PE Cy7, CD11b-PerCP Cy5.5, CD103- APC, PDCA1-AF488, Mar-1-efluor450, CD64-PE, TLR-4-APC, Dectin-1-PE, CD205- PerCPCy5.5, and CD206-BV785 antibodies, all from BioLegend (San Diego, CA); CD80- PECy5, CD86-605NC, and DC-SIGN-eFluor660 all from ThermoFisher Scientific, Waltham, MA); Dectin-2-AF647 (BioRad, Hercules, CA); and TLR 5-PE (Novus, Littleton, CO).

    Flow Cytometry:

    Article Title: LRP-1 Attenuates House Dust Mite-induced Eosinophilic Airway Inflammation by Suppressing Dendritic Cell-mediated Adaptive Immune Responses
    Article Snippet: Mouse DC subsets were identified and characterized by using anti- mouse Siglec F-PE-CF594, CD11c-APC Cy7, MHCII-PE Cy7, CD11b-PerCP Cy5.5, CD103- APC, PDCA1-AF488, Mar-1-efluor450, CD64-PE, TLR-4-APC, Dectin-1-PE, CD205- PerCPCy5.5, and CD206-BV785 antibodies, all from BioLegend (San Diego, CA); CD80- PECy5, CD86-605NC, and DC-SIGN-eFluor660 all from ThermoFisher Scientific, Waltham, MA); Dectin-2-AF647 (BioRad, Hercules, CA); and TLR 5-PE (Novus, Littleton, CO).

    Expressing:

    Article Title: LRP-1 Attenuates House Dust Mite-induced Eosinophilic Airway Inflammation by Suppressing Dendritic Cell-mediated Adaptive Immune Responses
    Article Snippet: Mouse DC subsets were identified and characterized by using anti- mouse Siglec F-PE-CF594, CD11c-APC Cy7, MHCII-PE Cy7, CD11b-PerCP Cy5.5, CD103- APC, PDCA1-AF488, Mar-1-efluor450, CD64-PE, TLR-4-APC, Dectin-1-PE, CD205- PerCPCy5.5, and CD206-BV785 antibodies, all from BioLegend (San Diego, CA); CD80- PECy5, CD86-605NC, and DC-SIGN-eFluor660 all from ThermoFisher Scientific, Waltham, MA); Dectin-2-AF647 (BioRad, Hercules, CA); and TLR 5-PE (Novus, Littleton, CO).

    MANN-WHITNEY:

    Article Title: LRP-1 Attenuates House Dust Mite-induced Eosinophilic Airway Inflammation by Suppressing Dendritic Cell-mediated Adaptive Immune Responses
    Article Snippet: Mouse DC subsets were identified and characterized by using anti- mouse Siglec F-PE-CF594, CD11c-APC Cy7, MHCII-PE Cy7, CD11b-PerCP Cy5.5, CD103- APC, PDCA1-AF488, Mar-1-efluor450, CD64-PE, TLR-4-APC, Dectin-1-PE, CD205- PerCPCy5.5, and CD206-BV785 antibodies, all from BioLegend (San Diego, CA); CD80- PECy5, CD86-605NC, and DC-SIGN-eFluor660 all from ThermoFisher Scientific, Waltham, MA); Dectin-2-AF647 (BioRad, Hercules, CA); and TLR 5-PE (Novus, Littleton, CO).



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    A) The percentage of CD11c+/Siglec F−/MHC IIhi total lung DCs and B) the percentage of lung DC monocyte-derived dendritic cells (moDCs), conventional myeloid dendritic cells (cDCs), CD103+ DCs, and plasmacytoid dendritic cells (pDCs) subsets as quantified by flow cytometry (P = NS, HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice, n= 9 mice per group, unpaired t test). C) Cell surface expression of <t>CD80</t> and CD86 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 8 – 9 mice per group, * P< 0.01, Mann-Whitney test). D) Cell surface expression of Dectin1, Dectin 2, CD205, CD206, and DC-SIGN by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 4 – 9 mice per group, * P = 0.029, Mann-Whitney test). E) Cell surface expression of TLR4 and TLR5 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n= 4 – 9 mice per group, *P < 0.05, unpaired t test).
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    Image Search Results


    A) The percentage of CD11c+/Siglec F−/MHC IIhi total lung DCs and B) the percentage of lung DC monocyte-derived dendritic cells (moDCs), conventional myeloid dendritic cells (cDCs), CD103+ DCs, and plasmacytoid dendritic cells (pDCs) subsets as quantified by flow cytometry (P = NS, HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice, n= 9 mice per group, unpaired t test). C) Cell surface expression of CD80 and CD86 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 8 – 9 mice per group, * P< 0.01, Mann-Whitney test). D) Cell surface expression of Dectin1, Dectin 2, CD205, CD206, and DC-SIGN by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 4 – 9 mice per group, * P = 0.029, Mann-Whitney test). E) Cell surface expression of TLR4 and TLR5 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n= 4 – 9 mice per group, *P < 0.05, unpaired t test).

    Journal: The Journal of allergy and clinical immunology

    Article Title: LRP-1 Attenuates House Dust Mite-induced Eosinophilic Airway Inflammation by Suppressing Dendritic Cell-mediated Adaptive Immune Responses

    doi: 10.1016/j.jaci.2017.10.044

    Figure Lengend Snippet: A) The percentage of CD11c+/Siglec F−/MHC IIhi total lung DCs and B) the percentage of lung DC monocyte-derived dendritic cells (moDCs), conventional myeloid dendritic cells (cDCs), CD103+ DCs, and plasmacytoid dendritic cells (pDCs) subsets as quantified by flow cytometry (P = NS, HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice, n= 9 mice per group, unpaired t test). C) Cell surface expression of CD80 and CD86 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 8 – 9 mice per group, * P< 0.01, Mann-Whitney test). D) Cell surface expression of Dectin1, Dectin 2, CD205, CD206, and DC-SIGN by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 4 – 9 mice per group, * P = 0.029, Mann-Whitney test). E) Cell surface expression of TLR4 and TLR5 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n= 4 – 9 mice per group, *P < 0.05, unpaired t test).

    Article Snippet: Mouse DC subsets were identified and characterized by using anti- mouse Siglec F-PE-CF594, CD11c-APC Cy7, MHCII-PE Cy7, CD11b-PerCP Cy5.5, CD103- APC, PDCA1-AF488, Mar-1-efluor450, CD64-PE, TLR-4-APC, Dectin-1-PE, CD205- PerCPCy5.5, and CD206-BV785 antibodies, all from BioLegend (San Diego, CA); CD80- PECy5, CD86-605NC, and DC-SIGN-eFluor660 all from ThermoFisher Scientific, Waltham, MA); Dectin-2-AF647 (BioRad, Hercules, CA); and TLR 5-PE (Novus, Littleton, CO).

    Techniques: Derivative Assay, Flow Cytometry, Expressing, MANN-WHITNEY

    Macrophage differentiation is enhanced by macrophage colony-stimulating factor (M-CSF) produced by IL-1β– or tumor necrosis factor (TNF)-α–treated first-trimester decidual cells (FTDCs). Peripheral monocytes were differentiated in conditioned medium [from FTDCs treated with control (EM; A), IL-1β (EMI; B) or TNF-α (EMT; C)] for 7 days with or without preincubation with an anti-human M-CSF antibody (D and E). Flow cytometric analysis assessed the expression of CD80 (F and G) and CD163 on CD11b+ (H and I) macrophages. Arrows indicate cells with increased ruffles and pseudopods. Arrowheads indicate cells with fewer ruffles and pseudopods. Results are expressed as means ± SEM (F–I). n = 11 (F–I). ∗P < 0.05. Original magnification, ×400 (A–E). α-M, anti-M-CSF; Exp, expression.

    Journal: The American Journal of Pathology

    Article Title: Modulation of Decidual Macrophage Polarization by Macrophage Colony-Stimulating Factor Derived from First-Trimester Decidual Cells

    doi: 10.1016/j.ajpath.2015.12.021

    Figure Lengend Snippet: Macrophage differentiation is enhanced by macrophage colony-stimulating factor (M-CSF) produced by IL-1β– or tumor necrosis factor (TNF)-α–treated first-trimester decidual cells (FTDCs). Peripheral monocytes were differentiated in conditioned medium [from FTDCs treated with control (EM; A), IL-1β (EMI; B) or TNF-α (EMT; C)] for 7 days with or without preincubation with an anti-human M-CSF antibody (D and E). Flow cytometric analysis assessed the expression of CD80 (F and G) and CD163 on CD11b+ (H and I) macrophages. Arrows indicate cells with increased ruffles and pseudopods. Arrowheads indicate cells with fewer ruffles and pseudopods. Results are expressed as means ± SEM (F–I). n = 11 (F–I). ∗P < 0.05. Original magnification, ×400 (A–E). α-M, anti-M-CSF; Exp, expression.

    Article Snippet: Then, macrophages were harvested and blocked with normal human IgG followed by staining with anti-human CD11B-PE, CD163-APC, and CD80-PECy5 (R&D Systems), according to the manufacturer's instruction.

    Techniques: Produced, Expressing